Anti-inflammatory effect of external use of escin on

ZHAO Shu-Qi , XU Shi-Qiang , CHENG Jing , CAO Xiao-Lu , ZHANG Ying , ZHOU Wei-Ping , HUANG Yan-Juan , WANG Jun  , HU Xia-Min

[ABSTRACT] Escin, as an internally applied anti-inflammatory agent, has been widely used in the treatment of inflammation and edema resulting from trauma or operation in the clinic. However, the effect of its external use on cutaneous inflammation and edema remains unexplored. In the present study, the anti-inflammatory and anti-edematous effects of external use of escin were studied in carrageenan-induced paw edema and histamine-induced capillary permeability in rats, paraxylene-induced ear swelling in mice, and cotton pellet-induced granuloma in rats. Effects of external use of escin gel on prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) were determined by ELISA ...

[ELISA for PGE2, TNF-α, and IL-1β analyses] The levels of PGE2, TNF-α and IL-1β were determined according to LI’s method  [1] . In brief, the tissue samples were obtained from paw edema and capillary permeability models,followed by homogenizing with ice-cold saline for a 10%(W/V) homogenate. The ELISA kits of PGE2 (JianglaiBio, Shanghai, China[1]), TNF-α (Lianke Bio, Wuhan, China) and IL-1β (Lianke Bio, Wuhan, China) were use, according to manufacturer’s instructions.

[备注] [1]“江莱生物”10年以来一直致力于免疫学技术的积累与沉淀，目前江莱生物实验室占地约3000平米，技术研发团队50余人，配备了全球最先进的分析和检测设备，我们通过自主创新以及与国内外的科研单位合作研发，不断突破新技术，目前我们研发了上千种指标，质量成熟，并长期出口到欧美市场，赢得了众多科研机构的认可 ...

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