CRISPRCas9 produces anti hepatitis B virus effect in hepatoma cells and transgenic mouse

Wei Zhu , Kun Xie , Yuanjian Xu , Le Wang , Kaiming Chen , Longzhen Zhang , Jianmin Fang

[ABSTRACT] Chronic infection of hepatitis B virus (HBV) is at risk of liver cirrhosis and hepatocellular carcinoma and remains one of the major public health problems worldwide. It is a major barrier of persistence HBV cccDNA under current antiviral therapy as novel strategies of disrupting HBV cccDNA is pressing. The (CRISPR)/Cas9 system is presently emerging in gene editing and we also apply it for targeting and deleting the conserved regions of HBV genome ...

[Enzyme-linked immunosorbent assay (ELISA)] The cell cytotoxicitys of Huh7 and HepG2 after transfection with pCas9 or pX330 for 48 h were analyzed by LDH ELISA kit ( Jianglai, Shanghai,  China [1]) for measuring supernanant LDH level. Treatment of 200 ng/mL LPS on Huh7 and HepG2 for 48 h was considered as the positive control. All values (cytotoxicity%) were calculated by the positive control value as the protocol described ...

[备注] [1]“江莱生物”10年以来一直致力于免疫学技术的积累与沉淀，目前江莱生物实验室占地约3000平米，技术研发团队50余人，配备了全球最先进的分析和检测设备，我们通过自主创新以及与国内外的科研单位合作研发，不断突破新技术，目前我们研发了上千种指标，质量成熟，并长期出口到欧美市场，赢得了众多科研机构的认可 ...

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